Rapid determination of the three-dimensional orientation of single molecules.
نویسنده
چکیده
A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light.
منابع مشابه
A three dimensional geometrical model for calculation of induced stresses surrounding longwall working
There are various criteria that need to be examined alongside each other when designing a longwall mining system. Challenges such as determination of the supported roof width, support system design, caving height determination, lateral or chain pillar size determination, and optimum support design for the main gate and tail gate roadways have to be tackled for this aim. Three-dimensional analys...
متن کاملCombined Unfolded Principal Component Analysis and Artificial Neural Network for Determination of Ibuprofen in Human Serum by Three-Dimensional Excitation–Emission Matrix Fluorescence Spectroscopy
This study describes a simple and rapid approach of monitoring ibuprofen (IBP). Unfolded principal component analysis-artificial neural network (UPCA-ANN) and excitation-emission spectra resulted from spectrofluorimetry method were combined to develop new model in the determination of IBF in human serum samples. Fluorescence landscapes with excitation wavelengths from 235 to 265 nm and emission...
متن کاملCombined Unfolded Principal Component Analysis and Artificial Neural Network for Determination of Ibuprofen in Human Serum by Three-Dimensional Excitation–Emission Matrix Fluorescence Spectroscopy
This study describes a simple and rapid approach of monitoring ibuprofen (IBP). Unfolded principal component analysis-artificial neural network (UPCA-ANN) and excitation-emission spectra resulted from spectrofluorimetry method were combined to develop new model in the determination of IBF in human serum samples. Fluorescence landscapes with excitation wavelengths from 235 to 265 nm and emission...
متن کاملDetermination of Principal Permeabilities in Two-Dimensional Fissured Rocks Using Permeability Circle (TECHNICAL NOTE)
Among the many parameters affecting the design of hydraulic structures on fissured rocks are orientation of principal permeability axes and degree of anisotropy of fissured rocks. Geological explorations rarely provide this type of information. If at all the information is available, methods employed are computationally cumbersome and inefficient. A new concept of simple equations and geometric...
متن کاملEFFECT OF SPIRAL DESIGN ON CRYSTAL ORIENTATION DURING SINGLE CRYSTAL GROWTH
Geometrical design of the spiral crystal selector can affect crystal orientation in the final single crystal structure. To achieve a better understanding of conditions associated with the onset of crystal orientation in a spiral crystal selector, temperature field was investigated using three-dimensional finite element method during the process. Different geometries of spiral crystal selec...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Optics letters
دوره 33 9 شماره
صفحات -
تاریخ انتشار 2001